Human Genetics with Mustard

Doug Wendell and Dawn Pickard, co-PIs
Oakland University, Rochester, Michigan

The goal of our project is to develop DNA markers for rapid-cycling Brassica rapa (aka Wisconsin Fast Plants) and educational materials that use these markers. Our target is college and advanced high school educators who can use our materials to teach key principles of genetics and the scientific process in general.

As we develop DNA markers and lab procedures we will post the here for all to try.

The purpose of this blog is to share information on the procedures and DNA markers that we develop for rapid cycling Brassica rapa (Fast Plants type). We will be posting protocols and data on the markers as we develop them. The intention is that this be interactive so we encourage users to post comments. Did the materials work? What steps did your students have problems with? What parts do you like? Do you have suggestions for improvement?

Please also check out our YouTube channel: http://www.youtube.com/user/HumanGeneticsMustard


Funding
This work was made possible by:
A pilot project grant from the Oakland University Teaching and Learning Committee
Educational Materials Development project grant from the National Science Foundation (grant # 0340910)
An ARRA Award from the National Institutes of Health (grant # 5 RC1 RR030293-02)

Friday, October 26, 2012

Possible Bias in Child Paternity



Based on what I have seen in my classes this fall,  I recommend that you always have multiple trios being mated in your lab class (rather than one mating in which you take lots of children).

In the mating of the two fathers to the mother, ideally there would be a 50/50 chance of any given Child being progeny of one or the other.  However, it looks like there tends to be bias in individual matings.  I have taken the extra seeds from the matings the students in my general biology lab did this fall and sprouted them.  Based on color markers, it appears that in any given mating most of the children come from one father, but which father is overrepresented is random.  In other words, sometimes most of the children are from Alleged Father #1 and other times most of the children are from Alleged Father #2.  If there are several matings going on in lab, then overall the students will see both possibilities.   However, if you did one paternity dispute and got lots of children, you probably wouldn’t get very satisfying results.

How is this happening?  I assume that even though the students collect pollen from both fathers on one swab, there is a tendency to get more pollen from one father (but which one appears to be random).  Either one is shedding more at the time of mating or they just spend a little more time on the anthers of one father than the other.