Human Genetics with Mustard

Doug Wendell and Dawn Pickard, co-PIs
Oakland University, Rochester, Michigan

The goal of our project is to develop DNA markers for rapid-cycling Brassica rapa (aka Wisconsin Fast Plants) and educational materials that use these markers. Our target is college and advanced high school educators who can use our materials to teach key principles of genetics and the scientific process in general.

As we develop DNA markers and lab procedures we will post the here for all to try.

The purpose of this blog is to share information on the procedures and DNA markers that we develop for rapid cycling Brassica rapa (Fast Plants type). We will be posting protocols and data on the markers as we develop them. The intention is that this be interactive so we encourage users to post comments. Did the materials work? What steps did your students have problems with? What parts do you like? Do you have suggestions for improvement?

Please also check out our YouTube channel: http://www.youtube.com/user/HumanGeneticsMustard


Funding
This work was made possible by:
A pilot project grant from the Oakland University Teaching and Learning Committee
Educational Materials Development project grant from the National Science Foundation (grant # 0340910)
An ARRA Award from the National Institutes of Health (grant # 5 RC1 RR030293-02)

Tuesday, June 21, 2011

DNA Markers

Information on DNA Markers

Working stock solution
10 µM primer in 5 mM Tris, pH 7.5


D1BrapaS1
Chromosome: 1

Primer Sequences
D1BrapaS1 F  ACGCTGTGATTTGTTGCTTCCGA
D1BrapaS1 R GGAGGAGCAAGCAGGACCAGGA

Size Range: 500 – 600 bp
Example Gel:



D9BrapaS1
Chromosome: 9

Primer Sequences
D9BrapaS1 F  CCAGCCAAATCGTCACTCATGCGA
D9BrapaS1 R TGCATGCCTAAGAGTTTGGAGTAACAC

Size Range: 400 – 600 bp

Example Gel:






D9BrapaS4
Chromosome: 9

Primer Sequences
D9BrapaS4 F  AGCGATGTAGCACCCGAGTCCA
D9BrapaS4 R TCGAGCTGAGAGGGAAGCTGTGA

Size Range: 300 – 600 bp

Example Gel:
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